Atoxigenic Aspergillus flavus (Aflasafe KE01) application reduces Fumonisin contamination in maize in lower Eastern Kenya

Abstract

Management of fumonisin contamination in maize has been a challenge largely because there are no effective management measures for Fusarium ear rots. Maize varieties that are resistant to fumonisin contamination are also lacking. This study was conducted to determine the efficacy of atoxigenic Aspergillus flavus (Aflasafe KE01) on fumonisin contamination of maize. The study was carried out in four sub-counties in lower Eastern Kenya. Twenty-four maize fields were selected in each sub-county; where 12 fields were treated with Aflasafe KE01, while 12 fields comprised the untreated controls. Aflasafe KE01 was applied at a rate of 5 kg/ha and 10kg/ha by hand broadcasting in maize fields two to three weeks before tasselling of maize. Fumonisin level in the maize samples was determined using Accuscan Pro-reader enzyme-linked immunosorbent assay (ELISA). The results showed that application of Aflasafe KE01 reduced fumonisin in the maize from the Aflasafe KE01 treated fields by up to 68% compared to samples from untreated fields. About 62.5% of the maize fields treated with 5kg/ha of Aflasafe KE01 met the European Commission regulatory threshold of ≤2ppm for total fumonisin as compared to about 45% from the control fields. This indicates that Aflasafe KE01 is a potential biopesticide for the management of fumonisin in maize. Therefore, efficacy of Aflasafe KE01 to reduce fumonisin contamination of other key staples in Kenya should also be evaluated.

Key words: Aflasafe KE01, atoxigenic Aspergillus sp., fumonisins, maize.